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KMID : 0665220230360010042
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Korean Journal of Food and Nutrition
2023 Volume.36 No. 1 p.42 ~ p.49
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Changes in Physicochemical Quality of the Extracts by Solvents in the Enzyme-Treated Abeliophyllum distichum Leaves
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Lee Kyung-Haeng
Jang Da-Bin
Lee Jae-Jun
Han Ki-Jung
Bae Kyung-Ah
Lee Won-Jong
Kwon Sun-Young
Lee Ho-Jin
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Abstract
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To enhance the efficacy of Abeliophyllum distichum leaves, extracts were prepared using different solvents for hydrolytic enzyme-treated Abeliophyllum distichum leaves. Physicochemical quality and antioxidant activity were measured. Soluble solids, reducing sugar, ascorbic acid, flavonoids, and polyphenols contents showed the lowest values in the control without enzyme treatment. However, they showed high contents in ethanol extract. In the case of enzyme treatment, their values were higher than those of the control. In particular, verbascoside content increased about 220 times more than that of the control group when treated with enzymes and extracted with 50% ethanol. pH was lowered upon enzymatic treatment. Regarding DPPH radical scavenging activity, for enzyme-free, 25% ethanol extract showed the highest activity among extracts with different solvents. For cellulase and pectinase-treated leaves, water extract showed the highest DPPH radical scavenging activity among extracts with different solvents. For leaves treated with enzyme combination, 50% ethanol extract showed the highest DPPH radical scavenging activity among extracts with different solvents. Regarding ABTS radical scavenging activity, it was generally higher in the 50% ethanol extract than in the water extract and 25% ethanol extract. In particular, verbascoside content was increased when the extract was prepared by co-treatment with enzymes and 50% ethanol.
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KEYWORD
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Abeliophyllum distichum leaves, enzyme treatment, physicochemical property, verbascoside, antioxidant activity
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